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| Frydman,Jacques Natan Grinapel; Fonseca,Adenilson de Souza da; Rocha,Vanessa Câmara da; Benarroz,Monica Oliveira; Rocha,Gabrielle de Souza; Pereira,Marcia de Oliveira; Pereira,Mario José; Medeiros,Aldo Cunha; Bernardo-Filho,Mario. |
| This work evaluated the effect of in vitro and in vivo treatment with ASA on the morphology of the red blood cells. Blood samples or Wistar rats were treated with ASA for one hour. Blood samples or animals treated with saline were used as control group. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of red blood cells were evaluated under optical microscopy. Data showed that the in vitro treatment for one hour with ASA at higher dose used significantly (p<0.05) modified the perimeter/area ratio of the red blood cells. No morphological alterations were obtained with the in vivo treatment. ASA use at highest doses could interfere on shape of red blood cells. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acetylsalicylic Acid; Blood Cells; Morphology. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300010 |
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| Costa,Maria Regina de Macedo; Ribeiro,Camila Godinho; Santos-Filho,Sebastião David; Neves,Rosane de Figueiredo; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario; Catanho,Maria Teresa Jansem de Almeida. |
| The development of experimental assays to study properties of herbal medicine is worthwhile. Vitex agnus castus (VAC) is utilized in popular medicine and some actions have been attributed to its extract. Blood cells (BC) and plasma proteins are labeled with technetium-99m (Tc-99m) and have been used in nuclear medicine, as in basic research. This procedure uses a reducing agent and stannous ion is utilized. There are reports that drugs can alter this labeling process. The aim of this work was to evaluate the influence of an aqueous extract of VAC on the labeling of blood constituents with Tc-99m. Blood was incubated with VAC, stannous chloride and Tc-99m, as sodium pertechnetate, and centrifuged. Samples of BC and plasma were separated, aliquots of BC and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oxidant properties; Plasma proteins; Red blood cells; Technetium-99m; Vitex agnus-castus. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600023 |
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| Giani,Tania Santos; Paoli,Severo de; Presta,Giuseppe Antonio; Maiworm,Adalgisa Ieda; Santos-Filho,Sebastião David; Brandão-Neto,José; Medeiros,Aldo da Cunha; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario. |
| Buzhong Yi Qi Wan (Buzhong) is a medicinal herb widely used in Traditional Chinese Medicine to treat the digestive and circulatory systems. Red blood cell and plasma proteins labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to investigate the effects of an aqueous Buzhong extract on the labeling of blood constituents with 99mTc. Heparinized blood (Wistar rats) was incubated in vitro with different Buzhong extract concentrations and 99mTc-labeling was performed. Plasma (P) and blood cells (BC) were separated and soluble (SF-P, SF-BC) and insoluble (IF-P, IF-BC) fractions were isolated. The radioactivity on blood constituents was determined and the percentage of incorporated radioactivity (%ATI) was calculated.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Technetium-99m; Red blood cell; Oxidant agent; Radiobiocomplex; Plasma proteins; Buzhong Yi Qi Wan. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600013 |
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| Missailidis,Sotiris; Perkins,Alan; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario. |
| In the continuous search for earlier diagnosis and improved therapeutic modalities against cancer, based on our constantly increasing knowledge of cancer biology, aptamers hold the promise to expand on current antibody success, but overcoming some of the problems faced with antibodies as therapeutic or delivery agents in cancer. However, as the first aptamer reached the market as an inhibitor against angiogenesis for the treatment of macular degeneration, aptamers have found only limited applications or interest in oncology, and even less as radiopharmaceuticals for diagnostic imaging and targeted radiotherapy of tumours. Yet, the chemistry for the labelling of aptamers and the options to alter their pharmacokinetic properties, to make them suitable for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aptamer; Radiopharmaceutical; Diagnostic imaging; Radiotherapy; Epithelial tumor. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700013 |
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| Moreno,Silvana Ramos Farias; Carvalho,Jorge José; Nascimento,Ana Lúcia; Pereira,Mário; Rocha,Emely Kazan; Diré,Gláucio; Arnobio,Adriano; Caldas,Luiz Querino de Araújo; Bernardo-Filho,Mario. |
| Many compounds affect the bioavailability of radiobiocomplexes as radiopharmaceuticals. Ginkgo Biloba extract (EGb) has several effects. The influence of an EGb on the bioavailability of the radiobiocomplex sodium pertechnetate (Na99mTcO4) and on the morphometry of the organs was evaluated. Rats were treated with EGb and Na99mTcO4 was injected. The animals were sacrificed; the radioactivity in the organs was counted. The results showed that EGb altered the Na99mTcO4 bioavailability in the kidneys, liver and duodenum. Morphometric analysis of the organs showed significant alterations (P<0.05), probably caused by metabolites generated by EGb and capable of altering the bioavailability of the Na99mTcO4. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ginkgo biloba extract; Bioavailability; Radiobiocomplex; Sodium pertechnetate; Morphometry. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700011 |
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| Ferreira-Machado,Samara C.; Salata,Camila; Rocha,Nazareth N.; Corrêa,Alexandre F.S.; Côrte-Real,Suzana; Peregrino,Antônio A.F.; Campos,Vera M.A. de; Andrade,Cherley B.V.; Bernardo-Filho,Mario; Cabral-Neto,Januário B.; Dealmeida,Carlos E.. |
| The caspase-3-cleaved presence was evaluated in this study in the heart of irradiated rats, during the decline of ventricular function. Female Wistar rats were irradiated with a single dose of radiation (15 Gy) delivered directly to the heart and the molecular, histological and physiological evaluations were performed at thirteen months post-irradiation. The expressions of procollagen type I, TGF-ß1 and caspase-3-cleaved were analyzed using Western blotting. Cardiac structural and functional alterations were investigated by echocardiography and electron microscopy. In the irradiated group, the levels of procollagen type I, TGF-ß1 and caspase-3-cleaved are increased. Significant histological changes (degeneration of heart tissue and collagen deposition) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; Caspase-3; Fibrosis; Heart; Ionizing radiation; TGF-β1. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100215 |
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| Pinto,Marcus Vinícius de Mello; Costa,Daniel Almeida da; Rocha,Lamara Laguardia Valente; Santos,Hélio Ricardo dos; Silva,André Luis dos Santos; Barbosa,Luis Guilherme; Reis,João Batista Alves dos; Bernardo-Filho,Mario. |
| This paper aims to compare the therapeutic effect of the laser As-Ga of 904nm and pulsed Ultrasound of 1 MHz applied in striated skeletal muscle of inflamed rats. The animals received an intramuscular injection of bupivacaine hydrochloride in tibialis muscle in order to induce the inflammatory process, and after 24 hours, the time was considered 0 for the initiation of therapy, using a laser and ultrasound. Samples collected the muscles of the animals were stained with Hematoxylin-Eosin and histological sections of the groups used for the analysis of the muscle tissue in relation to reducing the inflammatory process, comparing the results of the two therapies used. In this study it is suggested that both treatment with laser as with ultrasound can act as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lasers; Ultrasound; Inflammation; Rats. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700037 |
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| Meyer,Patricia Froes; Santos-Filho,Sebastião David; Ronzio,Oscar Ariel; Bonelli,Ludmila; Fonseca,Adenilson de Souza da; Costa,Iris do Ceu Clara; Brandão Neto,José; Medeiros,Aldo da Cunha; Bernardo-Filho,Mario. |
| Sources of magnetic field, radiofrequency and audible sonic waves and pulsed light have been used in physiotherapy to treat different disorders. In nuclear medicine, blood constituents(Bl-Co) are labeled with technetium-99m (99mTc) are used. This study evaluated the consequences of magnetic field, radiofrequency and audible sonic waves and intense pulsed light sources on the labeling of Bl-Co with 99mTc. Blood from Wistar rats was exposed to the cited sources. The labeling of Bl-Co with 99mTc was performed. Blood not exposed to the physical agents was used(controls). Data showed that the exposure to the different studied sources did not alter significantly (p>0.05) the labeling of Bl-Co. Although the results were obtained with animals, the data suggest... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood constituents; Magnetic field; Sonic and radiofrequency waves; Technetium-99m. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600014 |
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| Bernardo-Filho,Mario; Santos-Filho,Sebastião David; Moura,Egberto Gaspar de; Maiworm,Adalgisa Ieda; Orlando,Margarida Maria de Camões; Penas,Maria Expósito; Cardoso,Valbert Nascimento; Bernardo,Luciana Camargo; Brito,Lavínia de Carvalho. |
| Clinical images are worthwhile in Health Sciences and their analysis and correct interpretation aid the professionals,such as physicians, physiotherapists and occupational therapists, to make decisions and take subsequent therapeutic and/or rehabilitation measures. Other factors, besides the state of the disease, may interfere and affect the bioavailability of the radiopharmaceuticals (radiobiocomplexes) and the quality of the SPECT and PET images. Furthermore, the labeling of some of these radiobiocomplexes, such as plasma proteins, white blood cells and red blood cells, with 99mT, can also be modified. These factors include drugs (synthetic and natural) and dietary conditions, as well as some medical procedures (invasive or non-invasive), such as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drug interaction; Blood constituents; Radiobiocomplexes; Bioavailability. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700003 |
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| Rocha,Gabrielle de Souza; Pereira,Marcia de Oliveira; Benarroz,Mônica de Oliveira; Frydman,Jacques Natan Grinapel; Garcia-Pinto,Angélica Beatriz; Pereira,Mário José; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario. |
| This work evaluates effects of the sweetener with sucralose on the labeling of blood constituents with technetium-99m (99mTc), on the morphology of red blood cells (RBC) and on the biodistribution of sodium pertechnetate in Wistar rats. Animals were treated with sweetener for 8 days. Blood samples were withdrawn and the assay of labeling of blood constituents with 99mTc was performed. Blood cells (BC) and plasma (P) were isolated. Aliquots of BC and P were also precipitated, soluble and insoluble fractions separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the RBC was evaluated under optical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biodistribution; Blood; Morphology; Sweetener; Technetium-99m. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700021 |
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| Benarroz,Mônica Oliveira; Rocha,Gabrielle de Souza; Pereira,Márcia Oliveira; Geller,Mauro; Fonseca,Adenilson de Souza da; Presta,Giuseppe Antonio; Bernardo-Filho,Mario. |
| The aim of this study was to evaluate the effect of in vivo treatment with an aqueous cinnamon extract on the labeling of blood constituents with 99mTc and on the morphology of red blood cells from Wistar rats. Animals were treated with cinnamon extract at different doses and for different periods of time. As controls, animals treated with 0.9% NaCl. Labeling of blood constituents with 99mTc was performed. Plasma, blood cells and insoluble fractions were isolated. Radioactivity in each fraction was counted and the percentage of radioactivity (%ATI) was calculated. Also, blood smears were prepared to morphological analysis of red blood cells from. Data showed that in vivo cinnamon extract did not significantly (p>0.05) modify the %ATI of blood... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood constituents; Cinnamomum zeylanicum; Technetium-99m. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700023 |
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| Pereira,Marcia de Oliveira; Rocha,Gabrielle de Souza; Lombardi,Simone dos Santos; Geller,Mauro; Pereira,Mário José; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario. |
| The aim of this work was to evaluate the effect of fenoprofen on the labeling of blood constituents with technetium-99m, on the morphology of red blood cells and on the plasmid DNA. Blood samples from Wistar rats were incubated with fenoprofen and the assay of labeling of blood constituents with technetium-99m (99mTc) was performed. Blood cells, plasma, soluble and insoluble fractions of blood cells and plasma were separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) was determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the red blood cells (RBC) was evaluated. Plasmid (pBSK) was incubated with fenoprofen with stannous chloride, and agarose gel... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Technetium-99m; Blood; Morphology; Plasmid; Fenoprofen. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700022 |
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| Pinto,Nelson de Souza; Monteiro,Milena de Oliveira Bravo; Paiva,Dulciane Nunes; Santos-Filho,Sebastião David; Misssailidis,Sotiris; Thompson,Diane; Cabezuelo,Pedro Marín; Bernardo-Filho,Mario. |
| Among nonpharmacological strategy to manage fibromyalgia, exercise (aerobic) has shown efficacy. Whole-body vibration (WBV) exercise has been proposed as a potential clinical intervention. WBV would induce increase in growth hormone (GH). An impairment of the hypothalamic-pituitary-GH-Insulin Growth Factor-1(IGF-1) axis has been implicated in the pathophysiology of fibromyalgia. This article aims to review the studies on exploring the relationship between WBV and fibromyalgia. Literature searches were performed in the PubMed database on 04/03/2010 using terms related to "pain", "whole body vibration" and "fibromyalgia". An important number of publications were identified with the term "pain" and in comparison, only a small number of articles were found... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibromyalgia; Whole body vibration; PubMed. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100007 |
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